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Image Search Results
Journal:
Article Title: Engineering of the Fluorescent-Energy-Conversion Arm of Phi29 DNA Packaging Motor for Single-Molecule Studies
doi: 10.1002/smll.200900467
Figure Lengend Snippet: A) Concentration-dependent fluorescence intensity from the extended fluorescent arm of eGFP-gp16. B, a) Enlarged single-molecule image showing individual immobilized eGFP-gp16 distributed over the observed area (70 × 70 μm). b) Point-spread-function fitting of the circled fluorescent spot. The pixel size is 160 nm. C) Photobleaching step analysis showing a) one- or b) two-step drop from the immobilized eGFP-gp16. Time 0 indicates the beginning of laser excitation. D) Distribution of fluorescence intensity of the spots that showed one (solid bars) or two (shaded bars) steps in photobleaching, respectively. Xc represents the peak value obtained from each superimposed Gaussian plot over the data points, with Xc = 5.86 (solidline) for those spots containing one gp16 molecule and Xc = 10.53 (dashed line) for those containing two gp16 molecules.
Article Snippet: A
Techniques: Concentration Assay, Fluorescence